Kafarnik, C; McClellan, A; Dziasko, M; Daniels, JT; Guest, DJ; (2020) Canine Corneal Stromal Cells have multipotent mesenchymal stromal cell properties in vitro. Stem Cells and Development , 29 (7) pp. 425-439. 10.1089/scd.2019.0163.

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Abstract

The objective of this study was to determine whether corneal stromal cells (CSC) from the limbal and central corneal stroma in dogs have multipotent mesenchymal stromalem cell (MSC) properties, and whether this cell population can be differentiated into keratocyte-like cells (KDC). Normal, donated, mesocephalic dog corneas were used to isolate CSC in vitro. Immunhistochemistry (IHC) demonstrated a distinct population of CD90 expressing cells in the anterior stroma throughout the limbal and central cornea. CSC could be cultured from both the limbal and central cornea and the culture kinetics showed a progenitor cell profile. The CSC expressed stem cell markers CD90, CD73, CD105, N-cadherin and Pax6 whereas CD34 was negative. Limbal and central CSC differentiated into osteoblasts, chondrocytes and adipocytes confirming their multipotency. Co-culturing allogeneic peripheral blood mononuclear cells (PBMCss) with limbal CSCs did not affect baseline PBMC proliferation indicating a degree of innate immune privilege. Limbal CSC could be differentiated into keratocyte-like cells (KDCs) that expressed Kkeratocan, Llumican and ALDH1A3, and downregulated Pax6 and N-cadherin. In conclusion canine corneal stromal cells have multipotent mesenchymal stromal cell (MSC) properties similarly described in humans and could serve as a source of cells for cell therapy and studying corneal diseases.

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