Wang, GH;
Ma, KL;
Zhang, Y;
Hu, ZB;
Liu, L;
Lu, J;
Chen, PP;
... Liu, BC; + view all
(2019)
Caspase 3/ROCK1 pathway mediates high glucose-induced platelet microparticles shedding.
Biochemical and Biophysical Research Communications
, 509
(2)
pp. 596-602.
10.1016/j.bbrc.2018.12.166.
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BBRC-S-18-17410.pdf - Accepted Version Download (4MB) | Preview |
Abstract
Background: Platelet microparticles (PMPs) are closely associated with diabetic macrovascular complications. This study aimed to explore the underlying mechanisms of high glucose-induced PMPs generation. Methods: Washed platelets, obtained from the plasma of healthy male Sprague-Dawley rats, were incubated with high glucose. PMPs were isolated using gradient centrifugation and counted by flow cytometry. Expression and activity of ROCK1 and caspase3 were evaluated by real-time PCR, Western blotting, and activity assay kit. Results: High glucose enhanced PMPs shedding in the presence of collagen. The mRNA and protein levels of ROCK1, but not ROCK2, were increased in platelets incubated with high glucose. Y-27632, an inhibitor of ROCK, blocked the increased PMPs shedding induced by high glucose. Expression and activity of caspase3 were elevated in platelets under the high glucose conditions. Z-DVED-FMK, a caspase3 inhibitor, inhibited ROCK1 activity and decreased the PMPs generation under high glucose. Conclusion: High glucose increased PMPs shedding via caspase3-ROCK1 signal pathway.
| Type: | Article |
|---|---|
| Title: | Caspase 3/ROCK1 pathway mediates high glucose-induced platelet microparticles shedding |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.bbrc.2018.12.166 |
| Publisher version: | https://doi.org/10.1016/j.bbrc.2018.12.166 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biophysics, Platelet microparticles, High glucose, Rho-associated kinase 1, caspase3, ACTIVATION, THROMBIN, CYTOSKELETON, INVOLVEMENT, TYPE-1 |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Renal Medicine |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10090304 |
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