Diaz, JA;
Geard, A;
FitzPatrick, LM;
Delhove, JMKM;
Buckley, SMK;
Waddington, SN;
McKay, TR;
(2019)
Continual Conscious Bioluminescent Imaging in Freely Moving Mice.
Methods in Molecular Biology
, 2081
pp. 161-175.
10.1007/978-1-4939-9940-8_11.
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Continual Conscious Bioluminescent Imaging in Freely Moving Mice. Methods Mol Biol , 2081 pp..pdf - Accepted Version Download (523kB) | Preview |
Abstract
In vivo bioluminescent imaging allows the detection of reporter gene expression in rodents in real time. Here we describe a novel technology whereby we can generate somatotransgenic rodents with the use of a viral vector carrying a luciferase transgene. We are able to achieve long term luciferase expression by a single injection of lentiviral or adeno-associated virus vectors to newborn mice. Further, we describe whole body bioluminescence imaging of conscious mice in a noninvasive manner, thus enforcing the 3R’s (replacement, reduction, and refinement) of biomedical animal research.
| Type: | Article |
|---|---|
| Title: | Continual Conscious Bioluminescent Imaging in Freely Moving Mice |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-4939-9940-8_11 |
| Publisher version: | https://doi.org/10.1007/978-1-4939-9940-8_11 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Lentiviral vectors, Adeno-associated vectors, Transcription factor activated reporter, Biosensors, Luciferase, Neonatal mice, Luciferin, Bioluminescence imaging, Conscious imaging |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health > Maternal and Fetal Medicine |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10090105 |
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