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Robotic Extrusion of Algae-Laden Hydrogels for Large-Scale Applications

Malik, S; Hagopian, J; Mohite, S; Cao, L; Els, LS; Giannakopoulos, S; Beckett, R; ... Parker, B; + view all (2019) Robotic Extrusion of Algae-Laden Hydrogels for Large-Scale Applications. Global Challenges , Article 1900064. 10.1002/gch2.201900064. Green open access

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Abstract

A bioprinting technique for large‐scale, custom‐printed immobilization of microalgae is developed for potential applications within architecture and the built environment. Alginate‐based hydrogels with various rheology modifying polymers and varying water percentages are characterized to establish a window of operation suitable for layer‐by‐layer deposition on a large scale. Hydrogels formulated with methylcellulose and carrageenan, with water percentages ranging from 80% to 92.5%, demonstrate a dominant viscoelastic solid–like property with G′ > G″ and a low phase angle, making them the most suitable for extrusion‐based printing. A custom multimaterial pneumatic extrusion system is developed to be attached on the end effector of an industrial multiaxis robot arm, allowing precision‐based numerically controlled layered deposition of the viscous hydrogel. The relationship between the various printing parameters, namely air pressure, material viscosity, viscoelasticity, feed rate, printing distance, nozzle diameter, and the speed of printing, are characterized to achieve the desired resolution of the component. Printed prototypes are postcured in CaCl2 via crosslinking. Biocompatibility tests show that cells can survive for 21 days after printing the constructs. To demonstrate the methodology for scale‐up, a 1000 × 500 mm fibrous hydrogel panel is additively deposited with 3 different hydrogels with varying water percentages.

Type: Article
Title: Robotic Extrusion of Algae-Laden Hydrogels for Large-Scale Applications
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/gch2.201900064
Publisher version: https://doi.org/10.1002/gch2.201900064
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, additive manufacturing, hydrogels, immobilization, microalgae, photosynthetic, robotic extrusion, ALGINATE BEADS, WASTE-WATER, MICROALGAE, CELL, DESIGN, CENTRIFUGATION, IMMOBILIZATION, CLARIFICATION, PERFORMANCE, INTEGRATION
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of the Built Environment
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of the Built Environment > The Bartlett School of Architecture
URI: https://discovery.ucl.ac.uk/id/eprint/10086944
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