MacDonald, RB;
Randlett, O;
Oswald, J;
Yoshimatsu, T;
Franze, K;
Harris, WA;
(2015)
Müller glia provide essential tensile strength to the developing retina.
Journal of Cell Biology
, 210
(7)
pp. 1075-1083.
10.1083/jcb.201503115.
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Abstract
To investigate the cellular basis of tissue integrity in a vertebrate central nervous system (CNS) tissue, we eliminated Müller glial cells (MG) from the zebrafish retina. For well over a century, glial cells have been ascribed a mechanical role in the support of neural tissues, yet this idea has not been specifically tested in vivo. We report here that retinas devoid of MG rip apart, a defect known as retinoschisis. Using atomic force microscopy, we show that retinas without MG have decreased resistance to tensile stress and are softer than controls. Laser ablation of MG processes showed that these cells are under tension in the tissue. Thus, we propose that MG act like springs that hold the neural retina together, finally confirming an active mechanical role of glial cells in the CNS.
| Type: | Article |
|---|---|
| Title: | Müller glia provide essential tensile strength to the developing retina |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1083/jcb.201503115 |
| Publisher version: | https://doi.org/10.1083/jcb.201503115 |
| Language: | English |
| Additional information: | Copyright © 2015 MacDonald et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10085772 |
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