Xie, Y;
Maneas, E;
Islam, S;
Peveler, W;
Shapey, J;
Xia, W;
Ourselin, S;
... Vercauteren, T; + view all
(2019)
Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation.
In: Pogue, BW and Gioux, S, (eds.)
Molecular-Guided Surgery: Molecules, Devices, and Applications V.
SPIE: San Francisco, CA, USA.
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Abstract
Fluorescence-guided brain tumour resection, notably using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) for high-grade gliomas, has been demonstrated to provide better tissue differentiation, thereby improving patient outcomes when compared to white-light guidance. Novel fluorescence imaging devices aiming to increase detection specificity and sensitivity and targeting applications beyond high-grade gliomas are typically assessed by measurements using tissue-mimicking optical phantoms. The field currently lacks adequate phantoms with well-characterised tuneable optical properties. In this study, we developed soft tissue-mimicking fluorescence phantoms (TMFP) highly suitable for this purpose. We investigated: 1) the ability to independently tune optical and fluorescent properties; 2) the stability of the fluorescence signal over time; and 3) the potential of the proposed phantoms for imaging device validation. The TMFP is based on gel-wax which is an optically transparent mineral-oil based soft material. We embedded TiO2 as scattering material, carbon black oil-paint as background absorber, and CdTe Quantum Dots (QDs) as fluorophore because of its similar fluorescence spectrum to PpIX. Scattering and absorption properties were measured by a spectrophotometer, while the fluorescence was assessed by a wide-field fluorescence imaging system (WFFI) and a spectrometer. We demonstrated that: 1) the addition of QDs didn’t alter the phantom’s scattering which was only defined by the concentration of TiO2, whereas its absorption was defined by both QDs and colour oil paint; 2) the measured fluorescence intensity was linearlyproportional to the concentration of QDs; 3) the fluorescence intensity was stable over time (up to eight months); and 4) the fluorescence signal measured by the WFFI were strongly correlated to spectrometer measurements.
| Type: | Proceedings paper |
|---|---|
| Title: | Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation |
| Event: | SPIE BiOS |
| Location: | San Francisco, CA |
| Dates: | 02 February 2019 - 04 February 2019 |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1117/12.2508348 |
| Publisher version: | https://doi.org/10.1117/12.2508348 |
| Language: | English |
| Additional information: | This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10085658 |
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