Browne, JT;
Ng, Y;
Odlyha, M;
Gulabivala, K;
Bozec, L;
(2020)
Influence of root maturity or periodontal involvement on dentinal collagen changes following NaOCl irrigation: an ex-vivo study.
International Endodontic Journal
, 53
(1)
pp. 97-110.
10.1111/iej.13200.
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Abstract
Aims: To refine FTIR protocol for detection of NaOCl-induced dentinal collagen changes using an ex vivo irrigation model, and to apply it to determine the collagen change within 0.5mm of canal or root surfaces, with or without mature roots or periodontal involvement. // Methodology: Extracted human roots were irrigated with control saline (n=3) or 5% NaOCl (n=3) and sectioned into transverse discs for FTIR analyses, 0.5mm from both the canal lumen and root surface, before and after surface-treatment with 17% EDTA. Amide I/phosphate and amide II/phosphate absorbance ratios were compared using the Wilcoxon sign rank test. Mature roots without periodontal involvement were irrigated with: saline (n=7), 5% NaOCl (n=7), or 5% NaOCl+17% EDTA (n=7); those with periodontal involvement (n=7) or immature roots (n=7) were irrigated with 5% NaOCl. Dentine discs were then prepared for FTIR analyses. The effects of irrigant/root-maturity/ periodontal involvement were analysed using linear mixed models. // Results: FTIR analyses of the irrigated samples revealed significant (P < 0.05) reduction in collagen bands near the canal lumen after NaOCl irrigation using surface-EDTA treated samples. Irrigation with test solutions resulted in significant (P < 0.0001) dentinal collagen changes in the mature roots, whilst those in the immature roots were significantly (P < 0.05) greater compared with the mature roots with or without periodontal involvement; but there were no difference between the latter groups. // Conclusion: EDTA surface-treatment of polished dentine surfaces enhanced FTIR detection of NaOCl-induced collagen changes. Both root maturity and irrigation protocol influenced the ability of NaOCl to alter dentinal collagen up to 0.5mm from the canal lumen.
| Type: | Article |
|---|---|
| Title: | Influence of root maturity or periodontal involvement on dentinal collagen changes following NaOCl irrigation: an ex-vivo study |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/iej.13200 |
| Publisher version: | https://doi.org/10.1111/iej.13200 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | sodium hypochlorite, dentine, collagen, FTIR, root maturity, periodontal disease |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Restorative Dental Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10079352 |
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