Nimmo, IC;
Barbrook, AC;
Lassadi, I;
Chen, JE;
Geisler, K;
Smith, AG;
Aranda, M;
... Howe, CJ; + view all
(2019)
Genetic transformation of the dinoflagellate chloroplast.
eLife
, 8
, Article e45292. 10.7554/eLife.45292.
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Abstract
Coral reefs are some of the most important and ecologically diverse marine environments. At the base of the reef ecosystem are dinoflagellate algae, which live symbiotically within coral cells. Efforts to understand the relationship between alga and coral have been greatly hampered by the lack of an appropriate dinoflagellate genetic transformation technology. By making use of the plasmid-like fragmented chloroplast genome, we have introduced novel genetic material into the dinoflagellate chloroplast genome. We have shown that the introduced genes are expressed and confer the expected phenotypes. Genetically modified cultures have been grown for 1 year with subculturing, maintaining the introduced genes and phenotypes. This indicates that cells continue to divide after transformation and that the transformation is stable. This is the first report of stable chloroplast transformation in dinoflagellate algae
| Type: | Article |
|---|---|
| Title: | Genetic transformation of the dinoflagellate chloroplast |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.7554/eLife.45292 |
| Publisher version: | https://doi.org/10.7554/eLife.45292 |
| Language: | English |
| Additional information: | This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. https://creativecommons.org/licenses/by/4.0/ |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10079140 |
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