Nespovitaya, N;
Mahou, P;
Laine, RF;
Schierle, GSK;
Kaminski, CF;
(2017)
Heparin acts as a structural component of β-endorphin amyloid fibrils rather than a simple aggregation promoter.
Chemical Communications
, 53
(7)
pp. 1273-1276.
10.1039/c6cc09770g.
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Abstract
The aggregation promoter heparin is commonly used to study the aggregation kinetics and biophysical properties of protein amyloids. However, the underlying mechanism for amyloid promotion by heparin remains poorly understood. In the case of the neuropeptide β-endorphin that can reversibly adopt a functional amyloid form in nature, aggregation in the presence of heparin leads to a loss of function. Applying correlative optical super-resolution microscopy methods, we show that heparin incorporates into emerging β-endorphin fibrils forming an integral component and is essential for amyloid templating. This will have direct implications on β-endorphin's normal physiological function and raises concerns on the biological relevance of heparin-promoted amyloid models.
| Type: | Article |
|---|---|
| Title: | Heparin acts as a structural component of β-endorphin amyloid fibrils rather than a simple aggregation promoter |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/c6cc09770g |
| Publisher version: | http://dx.doi.org/10.1039/c6cc09770g |
| Language: | English |
| Additional information: | © The Royal Society of Chemistry 2017. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10078951 |
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