Huttunen, M;
Mercer, J;
(2019)
Quantitative PCR-Based Assessment of Vaccinia Virus RNA and DNA in Infected Cells.
Methods in Molecular Biology
, 2023
pp. 189-208.
10.1007/978-1-4939-9593-6_12.
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Abstract
Quantitative PCR-based methods have proven to be easy to use, cost-effective procedures for the quantification of viral gene expression and viral genome numbers. Quantitative PCR (qPCR) and quantitative reverse transcriptase-PCR (qRT-PCR) are rapid and sensitive approaches that can be used to pinpoint defects in viral DNA replication and transcriptional activity, respectively. Due to the significant nucleotide overlap between Poxviridae these methods can be employed across a wide range of viruses from this family. Here we provide methods for the quantification of vaccinia DNA replication by qPCR and quantification of the three classes of vaccinia gene transcription by qRT-PCR.
| Type: | Article |
|---|---|
| Title: | Quantitative PCR-Based Assessment of Vaccinia Virus RNA and DNA in Infected Cells |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-4939-9593-6_12 |
| Publisher version: | https://doi.org/10.1007/978-1-4939-9593-6_12 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Vaccinia gene expression, Vaccinia DNA replication, Quantitative PCR (qPCR), Quantitative reverse transcriptase PCR (qRT-PCR) |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10077758 |
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