Foglia, F;
Hazael, R;
Meersman, F;
Wilding, MC;
Sakai, VG;
Rogers, S;
Bove, LE;
... McMillan, PF; + view all
(2019)
In Vivo Water Dynamics in Shewanella oneidensis Bacteria at High Pressure.
Scientific Reports
, 9
, Article 8716. 10.1038/s41598-019-44704-3.
Preview |
Text
s41598-019-44704-3.pdf - Published Version Download (2MB) | Preview |
Abstract
Following observations of survival of microbes and other life forms in deep subsurface environments it is necessary to understand their biological functioning under high pressure conditions. Key aspects of biochemical reactions and transport processes within cells are determined by the intracellular water dynamics. We studied water difusion and rotational relaxation in live Shewanella oneidensis bacteria at pressures up to 500MPa using quasi-elastic neutron scattering (QENS). The intracellular difusion exhibits a signifcantly greater slowdown (by −10–30%) and an increase in rotational relaxation times (+10–40%) compared with water dynamics in the aqueous solutions used to resuspend the bacterial samples. Those results indicate both a pressure-induced viscosity increase and slowdown in ionic/macromolecular transport properties within the cells afecting the rates of metabolic and other biological processes. Our new data support emerging models for intracellular organisation with nanoscale water channels threading between macromolecular regions within a dynamically organized structure rather than a homogenous gel-like cytoplasm.
| Type: | Article |
|---|---|
| Title: | In Vivo Water Dynamics in Shewanella oneidensis Bacteria at High Pressure |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41598-019-44704-3 |
| Publisher version: | https://doi.org/10.1038/s41598-019-44704-3 |
| Language: | English |
| Additional information: | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10076752 |
Archive Staff Only
 |
View Item |
