Colin-York, HE;
Javanmardi, Y;
Barbieri, L;
Li, D;
Korobchevskaya, K;
Guo, Y;
Hall, C;
... Fritzsche, M; + view all
(2019)
Spatiotemporally Super-resolved Volumetric Traction Force Microscopy.
Nano Letters
, 19
(7)
pp. 4427-4434.
10.1021/acs.nanolett.9b01196.
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Abstract
Quantification of mechanical forces is a major challenge across biomedical sciences. Yet such measurements are essential to understanding the role of biomechanics in cell regulation and function. Traction force microscopy remains the most broadly applied force probing technology but typically restricts itself to single-plane two-dimensional quantifications with limited spatiotemporal resolution. Here, we introduce an enhanced force measurement technique combining 3D super-resolution fluorescence structural illumination microscopy and traction force microscopy (3D-SIMTFM) offering increased spatiotemporal resolution, openingup unprecedented insights into physiological three-dimensional force production in living cells.
| Type: | Article |
|---|---|
| Title: | Spatiotemporally Super-resolved Volumetric Traction Force Microscopy |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1021/acs.nanolett.9b01196 |
| Publisher version: | https://doi.org/10.1021/acs.nanolett.9b01196 |
| Language: | English |
| Additional information: | This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. https://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Traction force microscopy, super-resolution, 3D SIM, biomechanics, actin cytoskeleton |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10076646 |
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