Roubinet, C;
Tsankova, A;
Pham, TT;
Monnard, A;
Caussinus, E;
Affolter, M;
Cabernard, C;
(2017)
Spatio-temporally separated cortical flows and spindle geometry establish physical asymmetry in fly neural stem cells.
Nature Communications
, 8
, Article 1383. 10.1038/s41467-017-01391-w.
Preview |
Text
s41467-017-01391-w.pdf - Published Version Download (4MB) | Preview |
Abstract
Asymmetric cell division, creating sibling cells with distinct developmental potentials, can be manifested in sibling cell size asymmetry. This form of physical asymmetry occurs in several metazoan cells, but the underlying mechanisms and function are incompletely understood. Here we use Drosophila neural stem cells to elucidate the mechanisms involved in physical asymmetry establishment. We show that Myosin relocalizes to the cleavage furrow via two distinct cortical Myosin flows: at anaphase onset, a polarity induced, basally directed Myosin flow clears Myosin from the apical cortex. Subsequently, mitotic spindle cues establish a Myosin gradient at the lateral neuroblast cortex, necessary to trigger an apically directed flow, removing Actomyosin from the basal cortex. On the basis of the data presented here, we propose that spatiotemporally controlled Myosin flows in conjunction with spindle positioning and spindle asymmetry are key determinants for correct cleavage furrow placement and cortical expansion, thereby establishing physical asymmetry.
| Type: | Article |
|---|---|
| Title: | Spatio-temporally separated cortical flows and spindle geometry establish physical asymmetry in fly neural stem cells |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41467-017-01391-w |
| Publisher version: | https://doi.org/10.1038/s41467-017-01391-w |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Actomyosin, Animals, Animals, Genetically Modified, Brain, Cell Cycle, Cell Size, Chromatin, Drosophila Proteins, Drosophila melanogaster, Guanine Nucleotide Dissociation Inhibitors, Larva, Myosins, Neural Stem Cells, Spindle Apparatus, Tumor Suppressor Proteins |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10074825 |
Archive Staff Only
 |
View Item |
