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Identification and Isolation of Regulatory B Cells in Mouse and Human

Menon, M; Rosser, EC; Mauri, C; (2019) Identification and Isolation of Regulatory B Cells in Mouse and Human. Immunological Tolerance , 1899 pp. 55-66. 10.1007/978-1-4939-8938-6_5. Green open access

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Abstract

Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.

Type: Article
Title: Identification and Isolation of Regulatory B Cells in Mouse and Human
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-4939-8938-6_5
Publisher version: https://doi.org/10.1007/978-1-4939-8938-6_5
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: CD40, IL-10, Immature B cells, Inflammation, Regulatory B cells, T2-MZP B cells
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
URI: https://discovery.ucl.ac.uk/id/eprint/10072553
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