Menon, M;
Rosser, EC;
Mauri, C;
(2019)
Identification and Isolation of Regulatory B Cells in Mouse and Human.
Immunological Tolerance
, 1899
pp. 55-66.
10.1007/978-1-4939-8938-6_5.
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Abstract
Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.
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