Menon, M;
Rosser, EC;
Mauri, C;
(2019)
Identification and Isolation of Regulatory B Cells in Mouse and Human.
Immunological Tolerance
, 1899
pp. 55-66.
10.1007/978-1-4939-8938-6_5.
Preview |
Text
Menon, Rosser and Mauri, Method Chapter.pdf - Accepted Version Download (376kB) | Preview |
Preview |
Text
Menon, Rosser and Mauri, Method Chapter Figure 1.pdf - Accepted Version Download (353kB) | Preview |
Preview |
Text
Menon, Rosser and Mauri. Method Chapter FIgure 2.pdf - Accepted Version Download (297kB) | Preview |
Abstract
Regulatory B cells (Bregs) suppress immune response via the provision of IL-10. Due to the phenotypic heterogeneity of described Bregs, it is important to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell differentiation. In this chapter, we describe the methods necessary for the isolation of this subset of Bregs and their activation via CD40 in vitro.



1. | ![]() | 55 |
2. | ![]() | 32 |
3. | ![]() | 15 |
4. | ![]() | 8 |
5. | ![]() | 7 |
6. | ![]() | 6 |
7. | ![]() | 6 |
8. | ![]() | 6 |
9. | ![]() | 5 |
10. | ![]() | 5 |
Archive Staff Only
![]() |
View Item |