Steude, A;
Witts, EC;
Miles, GB;
Gather, MC;
(2016)
Arrays of microscopic organic LEDs for high-resolution optogenetics.
Science Advances
, 2
(5)
, Article e1600061. 10.1126/sciadv.1600061.
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Abstract
Optogenetics is a paradigm-changing new method to study and manipulate the behavior of cells with light. Following major advances of the used genetic constructs over the last decade, the light sources required for optogenetic control are now receiving increased attention. We report a novel optogenetic illumination platform based on high-density arrays of microscopic organic light-emitting diodes (OLEDs). Because of the small dimensions of each array element (6 × 9 μm2) and the use of ultrathin device encapsulation, these arrays enable illumination of cells with unprecedented spatiotemporal resolution. We show that adherent eukaryotic cells readily proliferate on these arrays, and we demonstrate specific light-induced control of the ionic current across the membrane of individual live cells expressing different optogenetic constructs. Our work paves the way for the use of OLEDs for cell-specific optogenetic control in cultured neuronal networks and for acute brain slices, or as implants in vivo.
| Type: | Article |
|---|---|
| Title: | Arrays of microscopic organic LEDs for high-resolution optogenetics |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1126/sciadv.1600061 |
| Publisher version: | https://doi.org/10.1126/sciadv.1600061 |
| Language: | English |
| Additional information: | Copyright © 2016, The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/), which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
| Keywords: | OLED, optogenetics, microdisplay, organic electronics, neuroscience, channelrhodopsin |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > The Sainsbury Wellcome Centre |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10071572 |
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