Davidson, IF;
Goetz, D;
Zaczek, MP;
Molodtsov, MI;
Huis In 't Veld, PJ;
Weissmann, F;
Litos, G;
... Peters, J-M; + view all
(2016)
Rapid movement and transcriptional re-localization of human cohesin on DNA.
The EMBO Journal
, 35
(24)
pp. 2671-2685.
10.15252/embj.201695402.
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Abstract
The spatial organization, correct expression, repair, and segregation of eukaryotic genomes depend on cohesin, ring-shaped protein complexes that are thought to function by entrapping DNA It has been proposed that cohesin is recruited to specific genomic locations from distal loading sites by an unknown mechanism, which depends on transcription, and it has been speculated that cohesin movements along DNA could create three-dimensional genomic organization by loop extrusion. However, whether cohesin can translocate along DNA is unknown. Here, we used single-molecule imaging to show that cohesin can diffuse rapidly on DNA in a manner consistent with topological entrapment and can pass over some DNA-bound proteins and nucleosomes but is constrained in its movement by transcription and DNA-bound CCCTC-binding factor (CTCF). These results indicate that cohesin can be positioned in the genome by moving along DNA, that transcription can provide directionality to these movements, that CTCF functions as a boundary element for moving cohesin, and they are consistent with the hypothesis that cohesin spatially organizes the genome via loop extrusion.
| Type: | Article |
|---|---|
| Title: | Rapid movement and transcriptional re-localization of human cohesin on DNA |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.15252/embj.201695402 |
| Publisher version: | https://doi.org/10.15252/embj.201695402 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Cell cycle, cohesin, genome organization, single‐molecule TIRF microscopy, transcription, CCCTC-Binding Factor, Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, DNA, Humans, Repressor Proteins, Single Molecule Imaging, Time Factors, Transcription, Genetic |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10068337 |
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