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Design, 3D printing and validation of a novel low-cost high-capacity sitting-drop bridge for protein crystallization

Hilton, ST; Kozielski, F; Penny, M; Talapatra, S; (2019) Design, 3D printing and validation of a novel low-cost high-capacity sitting-drop bridge for protein crystallization. Journal of Applied Crystallography , 52 pp. 171-174. 10.1107/S1600576718017545. Green open access

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Abstract

Sitting drop protein crystallization is not used as commonly as the hanging drop method for crystal optimization due to the limitations of commercially available sitting drop bridges, particularly when they are used in conjunction with 24-well crystallization plates. The commercially available sitting drop bridge, containing space for only a single drop, restricts their wider use. Proteins that preferentially crystallize under sitting-drop conditions therefore require more work, time and resources for their optimization. As a result of these limitations and using 3D-printing, we designed and developed a new sitting drop bridge where five crystallization drops can be placed simultaneously in each well of a 24-well crystallization plate. This significantly simplifies and increases the potential of sitting drops in crystal optimization, reducing costs and hence overcomes existing limitations of current approaches.

Type: Article
Title: Design, 3D printing and validation of a novel low-cost high-capacity sitting-drop bridge for protein crystallization
Open access status: An open access version is available from UCL Discovery
DOI: 10.1107/S1600576718017545
Publisher version: https://doi.org/10.1107/S1600576718017545
Language: English
Additional information: This version is the electronic reprint. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Protein crystallization, sitting drop, hanging drop, vapor-diffusion, microbridge, 3D-printing
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10066552
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