Hilton, ST;
Kozielski, F;
Penny, M;
Talapatra, S;
(2019)
Design, 3D printing and validation of a novel low-cost high-capacity sitting-drop bridge for protein crystallization.
Journal of Applied Crystallography
, 52
pp. 171-174.
10.1107/S1600576718017545.
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Abstract
Sitting drop protein crystallization is not used as commonly as the hanging drop method for crystal optimization due to the limitations of commercially available sitting drop bridges, particularly when they are used in conjunction with 24-well crystallization plates. The commercially available sitting drop bridge, containing space for only a single drop, restricts their wider use. Proteins that preferentially crystallize under sitting-drop conditions therefore require more work, time and resources for their optimization. As a result of these limitations and using 3D-printing, we designed and developed a new sitting drop bridge where five crystallization drops can be placed simultaneously in each well of a 24-well crystallization plate. This significantly simplifies and increases the potential of sitting drops in crystal optimization, reducing costs and hence overcomes existing limitations of current approaches.
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