Steven, RT;
Shaw, M;
Dexter, A;
Murta, T;
Green, FM;
Robinson, KN;
Gilmore, IS;
... Bunch, J; + view all
(2019)
Construction and testing of an atmospheric-pressure transmission-mode matrix assisted laser desorption ionisation mass spectrometry imaging ion source with plasma ionisation enhancement.
Analytica Chimica Acta
, 1051
pp. 110-119.
10.1016/j.aca.2018.11.003.
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Abstract
Matrix assisted laser desorption ionisation mass spectrometry (MALDI-MS) at atmospheric pressure (AP) is, with a few notable exceptions, overshadowed by its vacuum based forms and AP transmission mode (TM) MALDI-MS lacks the up-take its potential benefits might suggest. The reasons for this are not fully understood and it is clear further development is required to realise the flexibility and power of this ionisation method and geometry. Here we report the build of a new AP-TM-MALDI-MSI ion source with plasma ionisation enhancement. This novel ion source is used to analyse a selection of increasingly complex systems from molecular standards to murine brain tissue sections. Significant enhancement of detected ion intensity is observed in both positive and negative ion mode in all systems, with up to 2000 fold increases observed for a range of tissue endogenous species. The substantial improvements conferred by the plasma enhancement are then employed to demonstrate the acquisition of proof of concept tissue images, with high quality spectra obtained down to 10 × 10 μm pixel size.
| Type: | Article |
|---|---|
| Title: | Construction and testing of an atmospheric-pressure transmission-mode matrix assisted laser desorption ionisation mass spectrometry imaging ion source with plasma ionisation enhancement |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.aca.2018.11.003 |
| Publisher version: | https://doi.org/10.1016/j.aca.2018.11.003 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Matrix assisted laser desorption ionisation mass spectrometry, Plasma assisted ionisation, Transmission mode MALDI MS, Atmospheric pressure MALDI MS, Mass spectrometry imaging |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10065536 |
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