UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Characterization of regulatory T cells in obese omental adipose tissue in humans

Wu, D; Han, JM; Yu, X; Lam, AJ; Hoeppli, RE; Pesenacker, AM; Huang, Q; ... Levings, MK; + view all (2019) Characterization of regulatory T cells in obese omental adipose tissue in humans. European Journal of Immunology , 49 (2) pp. 336-347. 10.1002/eji.201847570. Green open access

[thumbnail of Wu-2018-Fat-Tregs-accepted16252729.pdf]
Preview
Text
Wu-2018-Fat-Tregs-accepted16252729.pdf - Accepted Version

Download (1MB) | Preview

Abstract

Obesity-associated visceral adipose tissue (AT) inflammation promotes insulin resistance and type 2 diabetes (T2D). In mice, lean visceral AT is populated with anti-inflammatory cells, notably regulatory T cells (Tregs) expressing the IL-33 receptor ST2. Conversely, obese AT contains fewer Tregs and more pro-inflammatory cells. In humans, however, there is limited evidence for a similar pattern of obesity-associated immunomodulation. We used flow cytometry and mRNA quantification to characterize human omental AT in 29 obese, 18 of whom had T2D. Patients with T2D had increased proportions of inflammatory cells, including M1 macrophages, with positive correlations to body mass index. In contrast, Treg frequencies negatively correlated to BMI but were comparable between T2D and non-T2D individuals. Compared to human thymic Tregs, omental AT Tregs expressed similar levels of FOXP3, CD25, IKZF2, and CTLA4, but higher levels of PPARG, CCR4, PRDM1, and CXCL2. ST2, however was not detectable on omental AT Tregs from lean or obese subjects. This is the first comprehensive investigation into how omental AT immunity changes with obesity and T2D in humans, revealing important similarities and differences to paradigms in mice. These data increase our understanding of how pathways of immune regulation could be targeted to ameliorate AT inflammation in humans.

Type: Article
Title: Characterization of regulatory T cells in obese omental adipose tissue in humans
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/eji.201847570
Publisher version: https://doi.org/10.1002/eji.201847570
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: IL-33, Regulatory T cells (Tregs), adipose tissue, immune regulation, metabolism, type 2 diabetes (T2D)
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/10065141
Downloads since deposit
Loading...
0Downloads
Download activity - last month
Loading...
Download activity - last 12 months
Loading...
Downloads by country - last 12 months
1.United States
6
2.Israel
2
3.China
2
4.India
1
5.Greece
1
6.Indonesia
1
7.Morocco
1
8.Uruguay
1
9.Russian Federation
1
10.Germany
1

Archive Staff Only

View Item View Item