Gardner, OFW;
Fahy, N;
Alini, M;
Stoddart, MJ;
(2017)
Joint mimicking mechanical load activates TGFβ1 in fibrin-poly(ester-urethane) scaffolds seeded with mesenchymal stem cells.
Journal of Tissue Engineering and Regenerative Medicine
, 11
(9)
pp. 2663-2666.
10.1002/term.2210.
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Abstract
Transforming growth factor‐β1 (TGF‐β1) is widely used in an active recombinant form to stimulate the chondrogenic differentiation of mesenchymal stem cells (MSCs). Recently, it has been shown that the application of multiaxial load, that mimics the loading within diarthrodial joints, to MSCs seeded in to fibrin‐poly(ester‐urethane) scaffolds leads to the endogenous production and secretion of TGF‐β1 by the mechanically stimulated cells, which in turn drives the chondrogenic differentiation of the cells within the scaffold. The work presented in this short communication provides further evidence that the application of joint mimicking multiaxial load induces the secretion of TGF‐β1 by mechanically stimulated MSCs. The results of this work also show that joint‐like multiaxial mechanical load activates latent TGF‐β1 in response to loading in the presence or absence of cells; this activation was not seen in non‐loaded control scaffolds. Despite the application of mechanical load to scaffolds with different distributions/numbers of cells no significant differences were seen in the percentage of active TGF‐β1 quantified in the culture medium of scaffolds from different groups. The similar level of activation in scaffolds containing different numbers of cells, cells at different stages of differentiation or with different distributions of cells suggests that this activation results from the mechanical forces applied to the culture system rather than differences in cellular behaviour. These results are relevant when considering rehabilitation protocols after cell therapy or microfracture, for articular cartilage repair, where increased TGF‐β1 activation in response to joint mobilization may improve the quality of developing cartilaginous repair material.
| Type: | Article |
|---|---|
| Title: | Joint mimicking mechanical load activates TGFβ1 in fibrin-poly(ester-urethane) scaffolds seeded with mesenchymal stem cells |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/term.2210 |
| Publisher version: | https://doi.org/10.1002/term.2210 |
| Language: | English |
| Additional information: | © 2016 The Authors. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
| Keywords: | cartilage, regeneration, endogenous TGF, growth factor, mechanobiology, paracrine |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10064466 |
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