Dawson, NAJ;
Lam, AJ;
Cook, L;
Hoeppli, RE;
Broady, R;
Pesenacker, AM;
Levings, MK;
(2018)
An optimized method to measure human FOXP3+ regulatory T cells from multiple tissue types using mass cytometry.
European Journal of Immunology
, 48
(8)
pp. 1415-1419.
10.1002/eji.201747407.
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Abstract
We optimized a method to detect FOXP3 by mass cytometry and compared the resulting data to conventional flow cytometry. We also demonstrated the utility of the protocol to profile antigen‐specific Tregs from whole blood, or Tregs from tissues such as cord blood, thymus and synovial fluid.
| Type: | Article |
|---|---|
| Title: | An optimized method to measure human FOXP3+ regulatory T cells from multiple tissue types using mass cytometry |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/eji.201747407 |
| Publisher version: | https://doi.org/10.1002/eji.201747407 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | CyTOF, Flow cytometry, FOXP3, Human, Mass cytometry, Regulatory T cells |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10063597 |
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