de Sousa, FD;
Vasconselos, PD;
da Silva, AFB;
Mota, EF;
da Rocha Tomé, A;
Mendes, FRDS;
Gomes, AMM;
... Monteiro-Moreira, ACDO; + view all
(2019)
RETRACTED: Hydrogel and membrane scaffold formulations of Frutalin (breadfruit lectin) within a polysaccharide galactomannan matrix have potential for wound healing.
International Journal of Biological Macromolecules
, 121
pp. 429-442.
10.1016/j.ijbiomac.2018.10.050.
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Abstract
Plant lectins are carbohydrate-binding proteins, which can interact with cell surfaces to initiate anti-inflammatory pathways, as well as immunomodulatory functions. Here, we have extracted, purified and part-characterized the bioactivity of Jacalin, Frutalin, DAL and PNA, before evaluating their potential for wound healing in cultured human skin fibroblasts. Only Frutalin stimulated fibroblast migration in vitro, prompting further studies which established its low cytotoxicity and interaction with TLR4 receptors. Frutalin also increased p-ERK expression and stimulated IL-6 secretion. The in vivo potential of Frutalin for wound healing was then assessed in hybrid combination with the polysaccharide galactomannan, purified from Caesalpinia pulcherrima seeds, using both hydrogel and membrane scaffolds formulations. Physical-chemical characterization of the hybrid showed that lectin-galactomannan interactions increased the pseudoplastic behaviour of solutions, reducing viscosity and increasing Frutalin's concentration. Furthermore, infrared spectroscopy revealed -OH band displacement, likely caused by interaction of Frutalin with galactose residues present on galactomannan chains, while average membrane porosity was 100 μm, sufficient to ensure water vapor permeability. Accelerated angiogenesis and increased fibroblast and keratinocyte proliferation were observed with the optimal hybrid recovering the lesioned area after 11 days. Our findings indicate Frutalin as a biomolecule with potential for tissue repair, regeneration and chronic wound healing.
| Type: | Article |
|---|---|
| Title: | RETRACTED: Hydrogel and membrane scaffold formulations of Frutalin (breadfruit lectin) within a polysaccharide galactomannan matrix have potential for wound healing |
| Location: | Netherlands |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.ijbiomac.2018.10.050 |
| Publisher version: | https://doi.org/10.1016/j.ijbiomac.2018.10.050 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. - PLEASE NOTE: This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editors-in-Chief [details of the retraction notice, dated 4 January 2025, can be viewed at https://doi.org/10.1016/j.ijbiomac.2024.138942]. Concerns were raised after several instances of image duplication in Fig. 4A and Fig. 8 were found and commented on in PubPeer. The corresponding author responded to these concerns and provided the raw images used in the article. However, the Editor-in-Chief found the author's reply did not adequately address all the concerns. Although the Editor found the correct images for Fig. 8, the raw images used for each time-point in Fig. 4A were found to be repeated in each of the folders, meaning that the analyses made using these repeated images were unable to provide an accurate and reproducible outcome. Consequently, the Editor has decided to retract the article. |
| Keywords: | Biomaterials, Caesalpinia pulcherrima, Frutalin, TLR4 |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10061929 |
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