Loraine, J;
Heinz, E;
De Sousa Almeida, J;
Milevskyy, O;
Voravuthikunchai,, SP;
Srimanote, P;
Kiratisin, P;
... Taylor, P; + view all
(2018)
Complement susceptibility in relation to genome sequence of recent Klebsiella pneumoniae isolates from Thai hospitals.
mSphere
, 3
(6)
, Article e00537-18.
Preview |
Text
Taylor_pneumoniae Isolates from Thai Hospitals.pdf - Published Version Download (3MB) | Preview |
Abstract
The capacity to resist the bactericidal action of complement (C=) is a strong but poorly understood virulence trait in Klebsiella spp. Killing requires activation of one or more C= pathways, assembly of C5b-9 membrane attack complexes (MACs) on the surface of the outer membrane (OM), and penetration of MACs into the target bilayer. We interrogated whole-genome sequences of 164 Klebsiella isolates from three tertiary hospitals in Thailand for genes encoding surface-located macromolecules considered to play a role in determination of C= resistance. Most isolates (154/164) were identified as Klebsiella pneumoniae, and the collection conformed to previously established population structures and antibiotic resistance patterns. The distribution of sequence types (STs) and capsular (K) types were also typical of global populations. The majority (64%) of isolates were resistant to C=, and the remainder were either rapidly or slowly killed. All isolates carried genes encoding capsular polysaccharides (K antigens), which have been strongly linked to C= resistance. In contrast to previous reports, there were no differences in the amount of capsule produced by C=-resistant isolates compared to C=-susceptible isolates, nor was there any correlation between serum reactivity and the presence of hypermucoviscous capsules. Similarly, there were no correlations between the presence of genes specifying lipopolysaccharide O-side chains or major OM proteins. Some virulence factors were found more frequently in C=-resistant isolates but were considered to reflect clonal ST expansion. Thus, no single gene accounts for the C= resistance of the isolates sequenced in this study.
| Type: | Article |
|---|---|
| Title: | Complement susceptibility in relation to genome sequence of recent Klebsiella pneumoniae isolates from Thai hospitals |
| Open access status: | An open access version is available from UCL Discovery |
| Publisher version: | https://doi.org/10.1128/ mSphere.00537-18 |
| Language: | English |
| Additional information: | Copyright © 2018 Loraine et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
| Keywords: | Klebsiella pneumoniae, complement resistance, lipopolysaccharide, polysaccharide capsules, whole-genome sequencing |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10060208 |
Archive Staff Only
 |
View Item |
