Janus, J;
Kanber, B;
Mahbuba, W;
Beynon, C;
Ramnarine, KV;
Lambert, DG;
Samani, NJ;
... Kelly, ME; + view all
(2019)
A Preclinical Ultrasound Method for the Assessment of Vascular Disease Progression in Murine Models.
Ultrasound
, 27
(2)
pp. 85-93.
10.1177/1742271X18793919.
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Abstract
INTRODUCTION: The efficacy of preclinical ultrasound at providing a quantitative assessment of mouse models of vascular disease is relatively unknown. In this study, preclinical ultrasound was used in combination with a semi-automatic image processing method to track arterial distension alterations in mouse models of abdominal aortic aneurysm and atherosclerosis. METHODS: Longitudinal B-mode ultrasound images of the abdominal aorta were acquired using a preclinical ultrasound scanner. Arterial distension was assessed using a semi-automatic image processing algorithm to track vessel wall motion over the cardiac cycle. A standard, manual analysis method was applied for comparison. RESULTS: Mean arterial distension was significantly lower in abdominal aortic aneurysm mice between day 0 and day 7 post-onset of disease (p < 0.01) and between day 0 and day 14 (p < 0.001), while no difference was observed in sham control mice. Manual analysis detected a significant decrease (p < 0.05) between day 0 and day 14 only. Atherosclerotic mice showed alterations in arterial distension relating to genetic modification and diet. Arterial distension was significantly lower (p < 0.05) in Ldlr−/−(++/−−) mice fed high-fat western diet when compared with both wild type (++/++) mice and Ldlr−/−(++/−−) mice fed chow diet. The manual method did not detect a significant difference between these groups. CONCLUSIONS: Arterial distension can be used as an early marker for the detection of arterial disease in murine models. The semi-automatic analysis method provided increased sensitivity to differences between experimental groups when compared to the manual analysis method.
| Type: | Article |
|---|---|
| Title: | A Preclinical Ultrasound Method for the Assessment of Vascular Disease Progression in Murine Models |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1177/1742271X18793919 |
| Publisher version: | https://doi.org/10.1177/1742271X18793919 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Vessel wall-motion, animal models, diagnostic imaging, in vivo, high-frequency ultrasound |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10060068 |
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