Amorim, MJ; Cotobal, C; Duncan, C; Mata, J; (2010) Global coordination of transcriptional control and mRNA decay during cellular differentiation. Molecular Systems Biology , 6 (380) 10.1038/msb.2010.38.

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Text Amorim, Maria J., et al. "Global coordination of transcriptional control and mRNA decay during cellular differentiation." Molecular systems biology 6.1 (2010)- 380.pdf - Published Version Download (1MB) | Preview

Abstract

The function of transcription in dynamic gene expression programs has been extensively studied, but little is known about how it is integrated with RNA turnover at the genome‐wide level. We investigated these questions using the meiotic gene expression program of Schizosaccharomyces pombe. We identified over 80 transcripts that co‐purify with the meiotic‐specific Meu5p RNA‐binding protein. Their levels and half‐lives were reduced in meu5 mutants, demonstrating that Meu5p stabilizes its targets. Most Meu5p‐bound RNAs were also targets of the Mei4p transcription factor, which induces the transient expression of ∼500 meiotic genes. Although many Mei4p targets showed sharp expression peaks, Meu5p targets had broad expression profiles. In the absence of meu5, all Mei4p targets were expressed with similar kinetics, indicating that Meu5p alters the global features of the gene expression program. As Mei4p activates meu5 transcription, Mei4p, Meu5p and their common targets form a feed‐forward loop, a motif common in transcriptional networks but not studied in the context of mRNA decay. Our data provide insight into the topology of regulatory networks integrating transcriptional and posttranscriptional controls.

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