Argemi, X;
Matelska, D;
Ginalski, K;
Riegel, P;
Hansmann, Y;
Bloom, J;
Pestel-Caron, M;
... Prevost, G; + view all
(2018)
Comparative genomic analysis of Staphylococcus lugdunensis shows a closed pan-genome and multiple barriers to horizontal gene transfer.
BMC Genomics
, 19
, Article 621. 10.1186/s12864-018-4978-1.
Preview |
Text
Comparative genomic analysis of Staphylococcus lugdunensis shows a closed pan-genome and multiple barriers to horizontal gene transfer.pdf - Accepted Version Download (2MB) | Preview |
Abstract
Background Coagulase negative staphylococci (CoNS) are commensal bacteria on human skin. Staphylococcus lugdunensis is a unique CoNS which produces various virulence factors and may, like S. aureus, cause severe infections, particularly in hospital settings. Unlike other staphylococci, it remains highly susceptible to antimicrobials, and genome-based phylogenetic studies have evidenced a highly conserved genome that distinguishes it from all other staphylococci. Results We demonstrate that S. lugdunensis possesses a closed pan-genome with a very limited number of new genes, in contrast to other staphylococci that have an open pan-genome. Whole-genome nucleotide and amino acid identity levels are also higher than in other staphylococci. We identified numerous genetic barriers to horizontal gene transfer that might explain this result. The S. lugdunensis genome has multiple operons encoding for restriction-modification, CRISPR/Cas and toxin/antitoxin systems. We also identified a new PIN-like domain-associated protein that might belong to a larger operon, comprising a metalloprotease, that could function as a new toxin/antitoxin or detoxification system. Conclusion We show that S. lugdunensis has a unique genome profile within staphylococci, with a closed pan-genome and several systems to prevent horizontal gene transfer. Its virulence in clinical settings does not rely on its ability to acquire and exchange antibiotic resistance genes or other virulence factors as shown for other staphylococci.
| Type: | Article |
|---|---|
| Title: | Comparative genomic analysis of Staphylococcus lugdunensis shows a closed pan-genome and multiple barriers to horizontal gene transfer |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1186/s12864-018-4978-1 |
| Publisher version: | https://doi.org/10.1186/s12864-018-4978-1 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Biotechnology & Applied Microbiology, Genetics & Heredity, Staphylococcus lugdunensis, Comparative genomics, Pan genome, Core genome, Toxin/antitoxin, Restriction-modification, CRISPR, COAGULASE-NEGATIVE STAPHYLOCOCCI, CRISPR-CAS SYSTEMS, RESTRICTION-MODIFICATION SYSTEMS, CLONAL POPULATION-STRUCTURE, SHORT PALINDROMIC REPEATS, TOXIN-ANTITOXIN SYSTEMS, PATHOGENIC BACTERIA, SEQUENCE-ANALYSIS, AUREUS, EVOLUTION |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10058973 |
Archive Staff Only
 |
View Item |
