Cárdenas-Fernández, M;
Hamley-Bennett, C;
Leak, DJ;
Lye, GJ;
(2018)
Continuous enzymatic hydrolysis of sugar beet pectin and l-arabinose recovery within an integrated biorefinery.
Bioresour Technol
, 269
pp. 195-202.
10.1016/j.biortech.2018.08.069.
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Abstract
Sugar beet pulp (SBP) fractionated by steam explosion, released sugar beet pectin (SB-pectin) which was selectively hydrolysed using a novel α-l-arabinofuranosidase (AF), yielding monomeric l-arabinose (Ara) and a galacturonic acid rich backbone (GABB). AF was immobilised on an epoxy-functionalised resin with 70% overall immobilisation yield. Pretreatment of SB-pectin, to remove coloured compounds, improved the stability of the immobilised AF, allowing its reutilisation for up to 10 reaction cycles in a stirred tank reactor. Continuous hydrolysis of SB-pectin was subsequently performed using a packed bed reactor (PBR) with immobilised AF. Reactor performance was evaluated using a Design of Experiment approach. Pretreated SB-pectin hydrolysis was run for 7 consecutive days maintaining 73% of PBR performance. Continuous separation of Ara from GABB was achieved by tangential flow ultrafiltration with 92% Ara recovery. These results demonstrate the feasibility of establishing a continuous bioprocess to obtain Ara from the inexpensive SBP biomass.
| Type: | Article |
|---|---|
| Title: | Continuous enzymatic hydrolysis of sugar beet pectin and l-arabinose recovery within an integrated biorefinery |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.biortech.2018.08.069 |
| Publisher version: | http://doi.org/10.1016/j.biortech.2018.08.069 |
| Language: | English |
| Additional information: | Copyright © 2018 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/). |
| Keywords: | Arabinofuranosidase, Biomass pretreatment, Continuous bioprocessing, Sugar beet pectin, l-Arabinose |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10056090 |
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