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Cell-wall remodeling drives engulfment during Bacillus subtiliss porulation

Ojkic, N; Lopez-Garrido, J; Pogliano, K; Endres, RG; (2016) Cell-wall remodeling drives engulfment during Bacillus subtiliss porulation. eLife , 5 , Article e18657. 10.7554/eLife.18657. Green open access

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Abstract

When starved, the Gram-positive bacterium Bacillus subtilis forms durable spores for survival. Sporulation initiates with an asymmetric cell division, creating a large mother cell and a small forespore. Subsequently, the mother cell membrane engulfs the forespore in a phagocytosis-like process. However, the force generation mechanism for forward membrane movement remains unknown. Here, we show that membrane migration is driven by cell wall remodeling at the leading edge of the engulfing membrane, with peptidoglycan synthesis and degradation mediated by penicillin binding proteins in the forespore and a cell wall degradation protein complex in the mother cell. We propose a simple model for engulfment in which the junction between the septum and the lateral cell wall moves around the forespore by a mechanism resembling the ‘template model’. Hence, we establish a biophysical mechanism for the creation of a force for engulfment based on the coordination between cell wall synthesis and degradation.

Type: Article
Title: Cell-wall remodeling drives engulfment during Bacillus subtiliss porulation
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/eLife.18657
Publisher version: http://dx.doi.org/10.7554/eLife.18657
Language: English
Additional information: © Copyright Ojkic et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Keywords: Science & Technology, Life Sciences & Biomedicine, Biology, Life Sciences & Biomedicine - Other Topics, Penicillin-Binding Proteins, Gene-Expression, Peptidoglycan Architecture, Forespore Engulfment, Spore Morphogenesis, Membrane Dynamics, Escherichia-Coli, Teichoic-Acid, Sporulation, Localization
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/10054227
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