Kargar, HM-P;
Azizi, H;
Mirnajafi-Zadeh, J;
Mani, AR;
Semnaniann, S;
(2018)
Orexin A presynaptically decreases inhibitory synaptic transmission in rat locus coeruleus neurons.
Neuroscience Letters
, 683
pp. 89-93.
10.1016/j.neulet.2018.06.022.
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Abstract
Locus coeruleus nucleus (LC) is a major noradrenergic nucleus in the brain. It receives dense orexinergic projections from lateral hypothalamus. Whilst it is known that orexin A increases firing rate of LC neurons, its effect on spontaneous and evoked inhibitory postsynaptic currents (sIPSCs and eIPSCs, respectively) has not been yet identified. In this research, we investigated the effect of orexin A on eIPSCs and sIPSCs in LC neurons. Whole-cell recordings revealed that orexin A suppresses eIPSCs amplitude in which this effect was blocked by an orexin type-1 receptors antagonist (SB-334867) and cannabinoid type-1 (CB1) receptors antagonist (AM251). Moreover, exposure of neurons to BAPTA (Ca2+ chelator) and U73122 (phospholipase C inhibitor) prevented orexin A-induced eIPSCs depression. On the other hand, orexin A increased pair pulse ratio and sIPSCs frequency but had no effect on sIPSCs amplitude. Our results revealed that eIPSCs suppression in the LC is mediated by CB1 receptor through a presynaptic mechanism.
| Type: | Article |
|---|---|
| Title: | Orexin A presynaptically decreases inhibitory synaptic transmission in rat locus coeruleus neurons |
| Location: | Ireland |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.neulet.2018.06.022 |
| Publisher version: | https://doi.org/10.1016/j.neulet.2018.06.022 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | AM251, Locus coeruleus nucleus, Orexin A, SB-334867, eIPSC, patch clamp recording, sIPSC |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Department of Education |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10051844 |
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