Wiegmann, V;
Martinez, CB;
Baganz, F;
(2018)
A simple method to determine evaporation and compensate for liquid losses in small-scale cell culture systems.
Biotechnology Letters
, 40
(7)
pp. 1029-1036.
10.1007/s10529-018-2556-x.
Preview |
Text
10.1007_s10529-018-2556-x.pdf - Published Version Download (924kB) | Preview |
Abstract
Objectives: Establish a method to indirectly measure evaporation in microwell-based cell culture systems and show that the proposed method allows compensating for liquid losses in fed-batch processes. Results: A correlation between evaporation and the concentration of Na + was found (R 2 = 0.95) when using the 24-well-based miniature bioreactor system (micro-Matrix) for a batch culture with GS-CHO. Based on these results, a method was developed to counteract evaporation with periodic water additions based on measurements of the Na + concentration. Implementation of this method resulted in a reduction of the relative liquid loss after 15 days of a fed-batch cultivation from 36.7 ± 6.7% without volume corrections to 6.9 ± 6.5% with volume corrections. Conclusion: A procedure was established to indirectly measure evaporation through a correlation with the level of Na + ions in solution and deriving a simple formula to account for liquid losses.
| Type: | Article |
|---|---|
| Title: | A simple method to determine evaporation and compensate for liquid losses in small-scale cell culture systems |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s10529-018-2556-x |
| Publisher version: | http://doi.org/10.1007/s10529-018-2556-x |
| Language: | English |
| Additional information: | Copyright © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
| Keywords: | Evaporation, Fed batch, GS-CHO cells, Miniature shaken bioreactor, Reproducibility |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10048698 |
Archive Staff Only
 |
View Item |
