Birkholz, O;
Burns, JR;
Richter, CP;
Psathaki, OE;
Howorka, S;
Piehler, J;
(2018)
Multi-functional DNA nanostructures that puncture and remodel lipid membranes into hybrid materials.
Nature Communications
, 9
, Article 1521. 10.1038/s41467-018-02905-w.
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Abstract
Altmetric: 12More detail Article | OPEN Multi-functional DNA nanostructures that puncture and remodel lipid membranes into hybrid materials Oliver Birkholz, Jonathan R. Burns, Christian P. Richter, Olympia E. Psathaki, Stefan Howorka & Jacob Piehler Nature Communicationsvolume 9, Article number: 1521 (2018) doi:10.1038/s41467-018-02905-w Download Citation DNA nanostructuresMembrane biophysicsNanoscale biophysicsSynthetic biology Received: 24 April 2017 Accepted: 08 January 2018 Published: 18 April 2018 Abstract Synthetically replicating key biological processes requires the ability to puncture lipid bilayer membranes and to remodel their shape. Recently developed artificial DNA nanopores are one possible synthetic route due to their ease of fabrication. However, an unresolved fundamental question is how DNA nanopores bind to and dynamically interact with lipid bilayers. Here we use single-molecule fluorescence microscopy to establish that DNA nanopores carrying cholesterol anchors insert via a two-step mechanism into membranes. Nanopores are furthermore shown to locally cluster and remodel membranes into nanoscale protrusions. Most strikingly, the DNA pores can function as cytoskeletal components by stabilizing autonomously formed lipid nanotubes. The combination of membrane puncturing and remodeling activity can be attributed to the DNA pores’ tunable transition between two orientations to either span or co-align with the lipid bilayer. This insight is expected to catalyze the development of future functional nanodevices relevant in synthetic biology and nanobiotechnology.
| Type: | Article |
|---|---|
| Title: | Multi-functional DNA nanostructures that puncture and remodel lipid membranes into hybrid materials |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41467-018-02905-w |
| Publisher version: | http://dx.doi.org/10.1038/s41467-018-02905-w |
| Language: | English |
| Additional information: | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| Keywords: | Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, Polymer-Supported Membranes, Protein-Protein Interactions, Single-Particle Tracking, Transmembrane Proteins, Mitochondrial Cristae, Organization, Curvature, Nanotubes, Nanopores, Localization |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10048039 |
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