Cristante, E;
Liyanage, SE;
Sampson, RD;
Kalargyrou, A;
De Rossi, G;
Rizzi, M;
Hoke, J;
... Bainbridge, JWB; + view all
(2018)
Late neuroprogenitors contribute to normal retinal vascular development in aHif2a-dependent manner.
Development
, 145
(8)
, Article dev157511. 10.1242/dev.157511.
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Abstract
In the adult central nervous system, endothelial and neuronal cells engage in tight cross-talk as key components of the so-called neurovascular unit. Impairment of their critical relationship adversely affects tissue homeostasis, as observed in neurodegenerative conditions including Alzheimer's and Parkinson's disease. In development, the influence of neuroprogenitor cells on angiogenesis is poorly understood. Here, we show that these cells interact intimately with the growing retinal vascular network, and we identify a novel regulatory mechanism of vasculature development mediated by hypoxia-inducible factor 2a (Hif2a). ByCre-loxgene excision, we show thatHif2ain retinal neuroprogenitor cells upregulates the expression of the pro-angiogenic mediators vascular endothelial growth factor and erythropoietin, whereas it locally downregulates the angiogenesis inhibitor endostatin. Importantly, absence ofHif2ain retinal neuroprogenitor cells causes a marked reduction of proliferating endothelial cells at the angiogenic front. This results in delayed retinal vascular development, fewer major retinal vessels and reduced density of the peripheral deep retinal vascular plexus. Our findings demonstrate that retinal neuroprogenitor cells are a critical component of the developing neurovascular unit.
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