Theisen, U;
Hennig, C;
Ring, T;
Schnabel, R;
Köster, RW;
(2018)
Neurotransmitter-mediated activity spatially controls neuronal migration in the zebrafish cerebellum.
PLoS Biology
, 16
(1)
, Article e2002226. 10.1371/journal.pbio.2002226.
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Abstract
Neuronal migration during embryonic development contributes to functional brain circuitry. Many neurons migrate in morphologically distinct stages that coincide with differentiation, requiring tight spatial regulation. It had been proposed that neurotransmitter-mediated activity could exert this control. Here, we demonstrate that intracellular calcium transients occur in cerebellar neurons of zebrafish embryos during migration. We show that depolarization increases and hyperpolarization reduces the speed of tegmental hindbrain neurons using optogenetic tools and advanced track analysis optimized for in vivo migration. Finally, we introduce a compound screening assay to identify acetylcholine (ACh), glutamate, and glycine as regulators of migration, which act regionally along the neurons’ route. We summarize our findings in a model describing how different neurotransmitters spatially interact to c ontrol neuronal migration. The high evolutionary conservation of the cerebellum and hindbrain makes it likely that polarization state-driven motility constitutes an important principle in building a functional brain.
| Type: | Article |
|---|---|
| Title: | Neurotransmitter-mediated activity spatially controls neuronal migration in the zebrafish cerebellum |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1371/journal.pbio.2002226 |
| Publisher version: | https://doi.org/10.1371/journal.pbio.2002226 |
| Language: | English |
| Additional information: | Copyright © 2018 Theisen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Statistical Science |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10044546 |
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