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Formation of supramolecular protein structures on gold surfaces

Domigan, LJ; Ashmead, H; Dimartino, S; Malmstrom, J; Pearce, FG; Blunt, M; Williams, DE; (2017) Formation of supramolecular protein structures on gold surfaces. Biointerphases , 12 (4) , Article 04E405. 10.1116/1.4986053. Green open access

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Abstract

Recent research has highlighted the exciting possibilities enabled by the use of protein structures as nanocomponents to form functional nanodevices. To this end, control over protein–protein and protein–surface interactions is essential. In this study, the authors probe the interaction of human peroxiredoxin 3 with gold surfaces, a protein that has been previously identified as having potential use in nanotechnology. Analytical ultracentrifugation and transmission electron microscopy revealed the pH mediated assembly of protein toroids into tubular structures across a small pH range. Quartz crystal microbalance with dissipation measurements showed differences in absorbed protein mass when pH is switched from pH 8.0 to 7.2, in line with the formation of supramolecular structures observed in solution studies. Scanning tunneling microscopy under ambient conditions showed that these protein tubes form on surfaces in a concentration dependent manner, with a tendency for protein adsorption and supramolecular assembly at the edges of Au(111) terraces. Finally, self-assembled monolayer modification of Au surfaces was explored as a means to control the adsorption and orientation of pH triggered protein structures.

Type: Article
Title: Formation of supramolecular protein structures on gold surfaces
Open access status: An open access version is available from UCL Discovery
DOI: 10.1116/1.4986053
Publisher version: https://doi.org/10.1116/1.4986053
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Technology, Biophysics, Materials Science, Biomaterials, Materials Science, Scanning-Tunneling-Microscopy, Self-Assembled Monolayer, Peroxiredoxin, Immobilization, Arrays, Nanostructures, Nanoparticles, Scaffolds, Chaperone, Shapes
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10039419
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