Shaw, M;
Bella, A;
Ryadnov, MG;
(2017)
CREIM: Coffee Ring Effect Imaging Model for Monitoring Protein Self-Assembly in Situ.
Journal of Physical Chemistry Letters
, 8
(19)
pp. 4846-4851.
10.1021/acs.jpclett.7b02147.
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Abstract
Protein self-assembly is fundamental to nanotechnology. Self-assembling structures are produced under static in vitro conditions typically forming over hours. In contrast, hydrodynamic intracellular environments employ far shorter time scales to compartmentalize highly concentrated protein solutions. Herein, we exploit the radial capillary flow within a drying sessile droplet (the coffee ring effect) to emulate dynamic native environments and monitor an archetypal protein assembly in situ using high-speed super-resolution imaging. We demonstrate that the assembly can be empirically driven to completion within minutes to seconds without apparent changes in supramolecular morphology. The model offers a reliable tool for the diagnosis and engineering of self-assembling systems under nonequilibrium conditions.
| Type: | Article |
|---|---|
| Title: | CREIM: Coffee Ring Effect Imaging Model for Monitoring Protein Self-Assembly in Situ |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1021/acs.jpclett.7b02147 |
| Publisher version: | http://dx.doi.org/10.1021/acs.jpclett.7b02147 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Physical Sciences, Technology, Chemistry, Physical, Nanoscience & Nanotechnology, Materials Science, Multidisciplinary, Physics, Atomic, Molecular & Chemical, Chemistry, Science & Technology - Other Topics, Materials Science, Physics, Structured Illumination Microscopy, Kinetics, Aggregation, Drops, Environments, Morphology, Pathways, Fibrils, Fiber, Flow |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10032970 |
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