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A functional role for small-conductance calcium-activated potassium channels in sensory pathways including nociceptive processes

Bahia, PK; Suzuki, R; Benton, DCH; Jowett, AJ; Chen, MX; Trezise, DJ; Dickenson, AH; (2005) A functional role for small-conductance calcium-activated potassium channels in sensory pathways including nociceptive processes. J NEUROSCI , 25 (14) 3489 - 3498. 10.1523/JNEUROSCI.0597-05.2005. Green open access

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Abstract

We investigated the role of small-conductance calcium-activated potassium (SK) and intermediate-conductance calcium-activated potassium channels in modulating sensory transmission from peripheral afferents into the rat spinal cord. Subunit-specific antibodies reveal high levels of SK3 immunoreactivity in laminas I, II, and III of the spinal cord. Among dorsal root ganglion neurons, both peripherin-positive (C-type) and peripherin-negative (A-type) cells show intense SK3 immunoreactivity. Furthermore, dorsal root-stimulated sensory responses recorded in vitro are inhibited when SK channel activity is increased with 1-ethyl-2-benzimidazolinone (1-EBIO). In vivo electrophysiological recordings show that neuronal responses to naturally evoked nociceptive and nonnociceptive stimuli increase after application of the selective SK channel blocker 8,14-diaza-1,7( 1,4)-diquinolinacyclotetradecaphanedium ditrifluoroacetate (UCL 1848), indicating that SK channels are normally active in moderating afferent input. Conversely, neuronal responses evoked by mechanical stimuli are inhibited when SK channel activity is increased with 1-EBIO. These effects are reversed by the subsequent application of UCL 1848. Our data demonstrate that SK channels have an important role in controlling sensory input into the spinal cord.

Type: Article
Title: A functional role for small-conductance calcium-activated potassium channels in sensory pathways including nociceptive processes
Open access status: An open access version is available from UCL Discovery
DOI: 10.1523/JNEUROSCI.0597-05.2005
Keywords: apamin, dorsal root ganglion, sensory transmission, afterhyperpolarization, C-fiber, K+ channel, CA2+-ACTIVATED K+ CHANNELS, ACTING MUSCLE-RELAXANT, ROOT GANGLION NEURONS, SPINAL-CORD INVITRO, ELECTROPHYSIOLOGICAL PROPERTIES, AFTER-HYPERPOLARIZATIONS, CL-SECRETION, SK CHANNELS, RAT, EXPRESSION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: https://discovery.ucl.ac.uk/id/eprint/9690
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