Soueid, A; Smith, T; Hall, S; Mudera, V; (2006) Pulmonary artery smooth muscle cells response to vaso-active stimulations in a real-time 3-Dimensional model. In: European Cells and Materials. (pp. 13 - 13).

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Abstract

INTRODUCTION: The tone of the pulmonary arteries is the summation of the activity of each smooth muscle cell (PASMC) within a vessel wall and its interaction with the endothelial cells and extracellular matrix (including collagen). There are reported phenotypic differences between PASMC in the inner & outer layers of pulmonary artery walls1. The response of a tissue engineered blood vessel to contractile and relaxing stimulants in-vitro is essential to predicting the response of the physiological and pathological vessels in-vivo. Previous work showed that pulmonary artery relaxation to nitric oxide is inhibited after exposure to chronic hypoxia 2. We hypothesised that PASMC will differ in their ability to contract or relax a 3D collagen gel. Using a Culture Force Monitor (CFM) we sought to quantify the cellular response of PASMC derived from inner and outer normal and hypoxic arteries, harvested from piglet models, over 24 hours in response to contractile agonists and relaxing antagonists.

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