Carey, MP;
Aniszewski, CA;
Fry, JP;
(1994)
Metabolism of progesterone in mouse brain.
The Journal of Steroid Biochemistry and Molecular Biology
, 50
(3-4)
pp. 213-217.
10.1016/0960-0760(94)90031-0.
Text
Fry_Carey%2C Aniszewski %26 Fry 1994.pdf Access restricted to UCL open access staff Download (445kB) |
Abstract
Incubation of whole mouse brain homogenate with [3H]progesterone resulted in two metabolites: the 5 alpha-reduced product, 5 alpha-pregnane-3,20-dione and another metabolite at a 3-fold greater yield. This differed from rat brain, which produced predominantly the 5 alpha-reduced metabolite under the same conditions. Subcellular fractionation of mouse brain demonstrated a particulate location for the 5 alpha-reduction of progesterone and a cytosolic location for the production of the unknown major metabolite. Treatment of this unknown metabolite with chromium trioxide resulted in a reconversion to progesterone, indicating the presence of a hydroxyl at position 3 or 20. Comparison of the chromatographic behaviour of the unknown metabolite with that of authentic progesterone derivatives suggested that this metabolite corresponds to 20-hydroxy-4-pregnene-3-one.
Type: | Article |
---|---|
Title: | Metabolism of progesterone in mouse brain |
Location: | ENGLAND |
DOI: | 10.1016/0960-0760(94)90031-0 |
Publisher version: | http://dx.doi.org/10.1016/0960-0760(94)90031-0 |
Language: | English |
Keywords: | Animals, Brain, Male, Mice, Mice, Inbred C57BL, Oxidation-Reduction, Progesterone, Progestins |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology |
URI: | https://discovery.ucl.ac.uk/id/eprint/57574 |
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