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Metabolism of progesterone in mouse brain

Carey, MP; Aniszewski, CA; Fry, JP; (1994) Metabolism of progesterone in mouse brain. The Journal of Steroid Biochemistry and Molecular Biology , 50 (3-4) pp. 213-217. 10.1016/0960-0760(94)90031-0.

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Abstract

Incubation of whole mouse brain homogenate with [3H]progesterone resulted in two metabolites: the 5 alpha-reduced product, 5 alpha-pregnane-3,20-dione and another metabolite at a 3-fold greater yield. This differed from rat brain, which produced predominantly the 5 alpha-reduced metabolite under the same conditions. Subcellular fractionation of mouse brain demonstrated a particulate location for the 5 alpha-reduction of progesterone and a cytosolic location for the production of the unknown major metabolite. Treatment of this unknown metabolite with chromium trioxide resulted in a reconversion to progesterone, indicating the presence of a hydroxyl at position 3 or 20. Comparison of the chromatographic behaviour of the unknown metabolite with that of authentic progesterone derivatives suggested that this metabolite corresponds to 20-hydroxy-4-pregnene-3-one.

Type: Article
Title: Metabolism of progesterone in mouse brain
Location: ENGLAND
DOI: 10.1016/0960-0760(94)90031-0
Publisher version: http://dx.doi.org/10.1016/0960-0760(94)90031-0
Language: English
Keywords: Animals, Brain, Male, Mice, Mice, Inbred C57BL, Oxidation-Reduction, Progesterone, Progestins
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: https://discovery.ucl.ac.uk/id/eprint/57574
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