UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Divalent metal inhibition of non-haem iron uptake across the rat duodenal brush border membrane

Smith, MW; Shenoy, KB; Debnam, ES; Dashwood, MR; Churchill, LJ; Srai, SK; (2002) Divalent metal inhibition of non-haem iron uptake across the rat duodenal brush border membrane. BRIT J NUTR , 88 (1) 51 - 56. 10.1079/BJN2002587. Green open access

[img]
Preview
PDF
download4.pdf
Available under License : See the attached licence file.

Download (954kB)

Abstract

Duodenal Fe2+ uptake is essential to body Fe2+ homeostasis, but the interaction of metals with the uptake process remains unclear. The present study compared the effects of four essential trace metals (Mn2+, Zn2+, Co2+ and Ni2+) with two toxic metals (Pb2+ and Cd2+) on Fe2+ uptake across the brush border membrane of villus-attached duodenal enterocytes. Everted rat duodenum was exposed to buffer containing 0.2 mm-Fe-59(2+)-ascorbate with or without the competing metal (2 mm) and the tissue was then processed for autoradiography allowing Fe2+ uptake to be determined at specific crypt-villus regions. The quantification method ensured that uptake by cells, rather than Fe2+ binding to the tissue surface, was measured. Fe2+ uptake was significantly inhibited by Cd2+ in upper villus enterocytes only and Pb2+ was without effect on Fe2+ uptake. The inhibition by Cd2+ was not due to general cell damage as judged by the release of lactate dehydrogenase from tissue into incubation fluid. Essential divalent trace metals reduced uptake significantly along the whole length of the crypt-villus axis. Cd2+ uptake, measured separately, took place at all regions of the villus-crypt axis, highest uptake being into crypt enterocytes. The very different uptake profiles for Cd2+ and Fe2+ suggests that the divalent metal transporter 1 is not the principal transporter of Cd2+. The addition of Fe2+ to incubation buffer inhibited Cd2+ uptake by both crypt and villus enterocytes. The possibility that the inhibitory actions of Fe2+ and Cd2+ on the uptakes of Cd2+ and Fe2+ respectively can be explained by a non-competitive action or the involvement of an additional metal transporter is discussed.

Type: Article
Title: Divalent metal inhibition of non-haem iron uptake across the rat duodenal brush border membrane
Open access status: An open access version is available from UCL Discovery
DOI: 10.1079/BJN2002587
Publisher version: http://dx.doi.org/10.1079/BJN2002587
Language: English
Additional information: © The Authors 2002
Keywords: intestinal iron transport, duodenum, cadmium uptake, brush border membrane, MOUSE DUODENUM, ABSORPTION, TRANSPORTER, CADMIUM, DMT1, EXPRESSION, ENTEROCYTES, DIET
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/185788
Downloads since deposit
227Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item