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A mechanism regulating the onset of Sox2 expression in the embryonic neural plate

Papanayotou, C; Mey, A; Birot, AM; Saka, Y; Boast, S; Smith, JC; Samarut, J; (2008) A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. PLOS Biology , 6 (1) , Article e2. 10.1371/journal.pbio.0060002. Green open access

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Abstract

In vertebrate embryos, the earliest definitive marker for the neural plate, which will give rise to the entire central nervous system, is the transcription factor Sox2. Although some of the extracellular signals that regulate neural plate fate have been identified, we know very little about the mechanisms controlling Sox2 expression and thus neural plate identity. Here, we use electroporation for gain-and loss-of-function in the chick embryo, in combination with bimolecular fluorescence complementation, two-hybrid screens, chromatin immunoprecipitation, and reporter assays to study protein interactions that regulate expression of N2, the earliest enhancer of Sox2 to be activated and which directs expression to the largest part of the neural plate. We show that interactions between three coiled-coil domain proteins (ERNI, Geminin, and BERT), the heterochromatin proteins HP1 alpha and HP1 gamma acting as repressors, and the chromatin-remodeling enzyme Brm acting as activator control the N2 enhancer. We propose that this mechanism regulates the timing of Sox2 expression as part of the process of establishing neural plate identity.

Type: Article
Title: A mechanism regulating the onset of Sox2 expression in the embryonic neural plate
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pbio.0060002
Publisher version: http://dx.doi.org/10.1371/journal.pbio.0060002
Language: English
Additional information: © 2008 Papanayotou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: SWI/SNF ATPASE BRG1, stem-cells, primitive streak, chicken sox2, placode development, functional-analysis, bmp inhibition, induction, xenopus, fgf
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/179351
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