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Lethal photosensitization of wound-associated microbes using indocyanine green and near-infrared light

Omar, GS; Wilson, M; Nair, SP; (2008) Lethal photosensitization of wound-associated microbes using indocyanine green and near-infrared light. BMC MICROBIOL , 8 , Article 111. 10.1186/1471-2180-8-111. Green open access

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Abstract

Background: The increase in resistance to antibiotics among disease-causing bacteria necessitates the development of alternative antimicrobial approaches such as the use of light-activated antimicrobial agents (LAAAs). Light of an appropriate wavelength activates the LAAA to produce cytotoxic species which can then cause bacterial cell death via loss of membrane integrity, lipid peroxidation, the inactivation of essential enzymes, and/or exertion of mutagenic effects due to DNA modification. In this study, the effect of the LAAA indocyanine green excited with high or low intensity light (808 nm) from a near-infrared laser (NIR) on the viability of Staphylococcus aureus, Streptococcus pyogenes and Pseudomonas aeruginosa was investigated.Results: All species were susceptible to killing by the LAAA, the bactericidal effect being dependent on both the concentration of indocyanine green and the light dose. Indocyanine green photosensitization using both high (1.37 W cm(-2)) and low (0.048 W cm(-2)) intensity NIR laser light was able to achieve reductions of 5.6 log(10) (> 99.99%) and 6.8 log(10) (> 99.99%) in the viable counts of Staph. aureus and Strep. pyogenes (using starting concentrations of 10(6) - 10(7) CFU ml(-1)). Kills of 99.99% were obtained for P. aeruginosa (initial concentration 10(8) - 10(9) CFU ml(-1)) photosensitized by the high intensity light (1.37 W cm(-2)); while a kill of 80% was achieved using low intensity irradiation (0.07 W cm(-2)). The effects of L-tryptophan (a singlet oxygen scavenger) and deuterium oxide (as an enhancer of the life span of singlet oxygen) on the survival of Staph. aureus was also studied. L-tryptophan reduced the proportion of Staph. aureus killed; whereas deuterium oxide increased the proportion killed suggesting that singlet oxygen was involved in the killing of the bacteria.Conclusion: These findings imply that indocyanine green in combination with light from a near-infrared laser may be an effective means of eradicating bacteria from wounds and burns.

Type: Article
Title: Lethal photosensitization of wound-associated microbes using indocyanine green and near-infrared light
Open access status: An open access version is available from UCL Discovery
DOI: 10.1186/1471-2180-8-111
Publisher version: http://www.biomedcentral.com/1471-2180/8/111
Language: English
Additional information: © 2008 Omar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: RETINAL-PIGMENT EPITHELIUM, PHOTODYNAMIC THERAPY, IN-VITRO, STAPHYLOCOCCUS-AUREUS, LASER THERAPY, GANGLION-CELLS, ACNE-VULGARIS, INFECTIONS, INACTIVATION, BACTERIA
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Microbial Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/168390
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