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Envoplakin, a novel precursor of the cornified envelope that has homology to desmoplakin

Ruhrberg, C; Hajibagheri, MAN; Simon, M; Dooley, TP; Watt, FM; (1996) Envoplakin, a novel precursor of the cornified envelope that has homology to desmoplakin. J CELL BIOL , 134 (3) 715 - 729. Green open access

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Abstract

The cornified envelope is a layer of transglutaminase cross-linked protein that is deposited under the plasma membrane of keratinocytes in the outermost layers of the epidermis. We present the sequence of one of the cornified envelope precursors, a protein with an apparent molecular mass of 210 kD. The 210-kD protein is translated from a 6.5-kb mRNA that is transcribed from a single copy gene. The mRNA was upregulated during suspension-induced terminal differentiation of cultured human keratinocytes. Like other envelope precursors, the 210-kD protein became insoluble in SDS and beta-mercaptoethanol on activation of transglutaminases in cultured keratinocytes. The protein was expressed in keratinizing and nonkeratinizing stratified squamous epithelia, but not in simple epithelia or nonepithelial cells, Immunofluorescence staining showed that in epidermal keratinocytes, both in vivo and in culture, the protein was upregulated during terminal differentiation and partially colocalized with desmosomal proteins. Immunogold EM confirmed the colocalization of the 210-kD protein and desmoplakin at desmosomes and on keratin filaments throughout the differentiated layers of the epidermis, Sequence analysis showed that the 210-kD protein is homologous to the keratin-binding proteins desmoplakin, bullous pemphigoid antigen 1, and plectin. These data suggest that the 210-kD protein may link the cornified envelope to desmosomes and keratin filaments. We propose that the 210-kD protein be named ''envoplakin.''

Type: Article
Title: Envoplakin, a novel precursor of the cornified envelope that has homology to desmoplakin
Open access status: An open access version is available from UCL Discovery
Keywords: BULLOUS PEMPHIGOID ANTIGEN, KERATIN INTERMEDIATE FILAMENTS, KIDNEY EPITHELIAL-CELLS, CROSS-LINKED ENVELOPE, HUMAN EPIDERMAL-CELLS, TERMINAL DIFFERENTIATION, SPATIAL REGULATION, DESMOSOMAL PLAQUE, PROTEIN-STRUCTURE, CULTURED-CELLS
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/16430
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