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Neuropilin-mediated neural crest cell guidance is essential to organise sensory neurons into segmented dorsal root ganglia

Schwarz, Q; Maden, CH; Davidson, K; Ruhrberg, C; (2009) Neuropilin-mediated neural crest cell guidance is essential to organise sensory neurons into segmented dorsal root ganglia. DEVELOPMENT , 136 (11) 1785 - 1789. 10.1242/dev.034322. Green open access

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Abstract

The peripheral nervous system (PNS) of higher vertebrates is segmented to align the spinal nerve roots with the vertebrae. This co-patterning is set up during embryogenesis, when vertebrae develop from the sclerotome layer of the metameric somites, and PNS neurons and glia differentiate from neural crest cells (NCCs) that preferentially migrate into the anterior sclerotome halves. Previous analyses of mice deficient in the class 3 semaphorin (SEMA3) receptors neuropilin (NRP) 1 or 2 raised the possibility that each controlled a distinct aspect of trunk NCC migration. We now demonstrate that both pathways act sequentially in distinct NCC subpopulations and thereby cooperate to enforce segmental NCC migration. Specifically, SEMA3A/NRP1 signalling first directs one population of NCCs from the intersomitic path into the sclerotome, and SEMA3F/NRP2 signalling acts subsequently to restrict a second population to the anterior half of the sclerotome. NCC exclusion from either the posterior sclerotome or the intersomitic boundary is sufficient to enforce the separation of neighbouring NCC streams and the segregation of sensory NCC progeny into metameric dorsal root ganglia (DRG). By contrast, the combined loss of both guidance pathways leads to ectopic invasion of the intersomitic furrows and posterior sclerotome halves, disrupting metameric NCC streaming and DRG segmentation.

Type: Article
Title: Neuropilin-mediated neural crest cell guidance is essential to organise sensory neurons into segmented dorsal root ganglia
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/dev.034322
Publisher version: http://www.ncbi.nlm.nih.gov/pmc/ articles/PMC26801...
Keywords: Dorsal root ganglia, Neural crest cell, Neuropilin, Segmentation, Semaphorin, Sensory neurons, Mouse, AXON GUIDANCE, SYMPATHETIC NEURONS, NERVOUS-SYSTEM, PROXIMAL RIBS, SEMAPHORIN, REQUIREMENT, DIFFERENTIATION, EXPRESSION, PLEXIN-A3, MIGRATION
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/16373
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