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Ultra scale-down approaches to enhance the creation of bioprocesses at scale: impacts of process shear stress and early recovery stages

Rayat, ACME; Chatel, A; Hoare, M; Lye, GJ; (2016) Ultra scale-down approaches to enhance the creation of bioprocesses at scale: impacts of process shear stress and early recovery stages. Current Opinion in Chemical Engineering , 14 pp. 150-157. 10.1016/j.coche.2016.09.012. Green open access

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Abstract

The sensitivity of biological materials to shear stress conditions encountered during large-scale bioprocessing makes successful scale up from the bench challenging. Ultra scale-down technologies seek to use just millilitre quantities to enhance our understanding of the impact of the process environment as a basis for process optimisation. They can help speed translation of new biological discoveries to market and reduce risks encountered in scale up. They are important both as process discovery tools and as preparative tools to yield material for study of subsequent stages. In this review the focus is on the early recovery stages post fermentation or cell culture and in particular the use of continuous-flow and dead-end centrifugation integrated with preparative stages (e.g. flocculation) and subsequent depth filtration. Examples range from therapeutic antibodies, to rationally engineered (synthetic biology) host strains, to stem cells for therapy.

Type: Article
Title: Ultra scale-down approaches to enhance the creation of bioprocesses at scale: impacts of process shear stress and early recovery stages
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.coche.2016.09.012
Publisher version: http://dx.doi.org/10.1016/j.coche.2016.09.012
Language: English
Additional information: Copyright © 2017 The Authors. Open Access: made available under a Creative Commons license (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/)
Keywords: Science & Technology, Life Sciences & Biomedicine, Technology, Biotechnology & Applied Microbiology, Engineering, Chemical, Engineering, Rec E. Coli, Clarification Performance, Antibody Fragments, Cell-suspensions, Domain Antibody, Centrifugation, Fermentation, Prediction, Precipitation, Purification
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1534476
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