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Digital PCR dynamic range is approaching that of real-time quantitative PCR

Jones, GM; Busby, E; Garson, JA; Grant, PR; Nastouli, E; Devonshire, AS; Whale, AS; (2016) Digital PCR dynamic range is approaching that of real-time quantitative PCR. Biomolecular Detection and Quantification , 10 pp. 31-33. 10.1016/j.bdq.2016.10.001. Green open access

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Abstract

Digital PCR (dPCR) has been reported to be more precise and sensitive than real-time quantitative PCR (qPCR) in a variety of models and applications. However, in the majority of commercially available dPCR platforms, the dynamic range is dependent on the number of partitions analysed and so is typically limited to four orders of magnitude; reduced compared with the typical seven orders achievable by qPCR. Using two different biological models (HIV DNA analysis and KRAS genotyping), we have demonstrated that the RainDrop Digital PCR System (RainDance Technologies) is capable of performing accurate and precise quantification over six orders of magnitude thereby approaching that achievable by qPCR.

Type: Article
Title: Digital PCR dynamic range is approaching that of real-time quantitative PCR
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.bdq.2016.10.001
Publisher version: http://dx.doi.org/10.1016/j.bdq.2016.10.001
Language: English
Additional information: Copyright © 2016 The Author(s). Published by Elsevier GmbH. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
URI: https://discovery.ucl.ac.uk/id/eprint/1534172
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