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Insertion Element IS6110 based characterisation of Nepalese tuberculosis strains into different genetic lineages

Moganeradj, K; Rajendram, D; Khadge, S; Sonnenberg, P; Abubakar, I; McHugh, TD; Macdonald, M; ... Arnold, C; + view all (2016) Insertion Element IS6110 based characterisation of Nepalese tuberculosis strains into different genetic lineages. Clinical Microbiology and Infectious Diseases , 1 (1) 10.15761/CMID.1000101. Green open access

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Abstract

Nepal is geographically located between India and China, a region containing significant Tuberculosis (TB) and Multi-Drug Resistance (MDR-TB) burdens. However, limited information is available on the phylogenetic diversity of Mycobacterium tuberculosis (Mtb) in Nepal. To gain further insight into the diversity of Mtb in Nepal, consecutive clinical samples from 176 newly diagnosed pulmonary tuberculosis patients were collected from two hospitals in Nepal. Insertion Site IS6110 Fluorescent Amplified Fragment Length Polymorphism (FAFLP) PCR and rpoB sequence analysis were carried out on genomic DNA extracts of cultured strains to assign them to accepted genetic lineages and identify MDR-TB. In this study, the IS6110 based characterisation showed a prevalence of 36.36% Central Asian Strain (CAS), 18.75% Beijing, 7.95 % Haarlem, 3.97% X, 2.2% each of Latin American Mediterranean (LAM), T-Uganda and T, 1.7% S and 24.4% were unassigned. Further, 3.9% of total M. tuberculosis isolates were of rifampicin resistant genotypes thus indicating that the prevalence of MDR could be higher than the country wide prevalence of MDR among new TB cases (2.2%) as reported by the national drug resistance survey carried out in 2011/2012.

Type: Article
Title: Insertion Element IS6110 based characterisation of Nepalese tuberculosis strains into different genetic lineages
Open access status: An open access version is available from UCL Discovery
DOI: 10.15761/CMID.1000101
Publisher version: http://doi.org/10.15761/CMID.1000101
Language: English
Additional information: ©2016 Kartyk M. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Tuberculosis; IS6110; FAFLP; Rifampicin resistance; TB lineages
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Institute for Global Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Institute for Global Health > Infection and Population Health
URI: https://discovery.ucl.ac.uk/id/eprint/1523865
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