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Nanoparticles in the lung and their protein corona: the few proteins that count

Whitwell, H; Mackay, R-M; Elgy, C; Morgan, C; Griffiths, M; Clark, H; Skipp, P; (2016) Nanoparticles in the lung and their protein corona: the few proteins that count. Nanotoxicology , 10 (9) pp. 1385-1394. 10.1080/17435390.2016.1218080. Green open access

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Abstract

The formation of protein coronae on nanoparticles (NPs) has been investigated almost exclusively in serum, despite the prevailing route of exposure being inhalation of airborne particles. In addition, an increasing number of nanomedicines, that exploit the airways as the site of delivery, are undergoing medical trials. An understanding of the effects of NPs on the airways is therefore required. To further this field, we have described the corona formed on polystyrene (PS) particles with different surface modifications and on titanium dioxide particles when incubated in human bronchoalveolar lavage fluid (BALF) from patients with pulmonary alveolar proteinosis (PAP). We show, using high-resolution quantitative mass spectrometry (MSE), that a large number of proteins bind with low copy numbers but that a few “core” proteins bind to all particles tested with high fidelity, averaging the surface properties of the different particles independent of the surface properties of the specific particle. The averaging effect at the particle surface means that differing cellular effects may not be due to the protein corona but due to the surface properties of the nanoparticle once inside the cell. Finally, the adherence of surfactant associated proteins (SP-A, B and D) suggests that there may be interactions with lipids and pulmonary surfactant (PSf), which could have potential in vivo health effects for people with chronic airway diseases such as asthma and chronic obstructive pulmonary disease (COPD), or those who have increased susceptibility toward other respiratory diseases.

Type: Article
Title: Nanoparticles in the lung and their protein corona: the few proteins that count
Open access status: An open access version is available from UCL Discovery
DOI: 10.1080/17435390.2016.1218080
Publisher version: http://dx.doi.org/10.1080/17435390.2016.1218080
Language: English
Additional information: This is an Accepted Manuscript of an article published by Taylor & Francis in Nanotoxicology on 18 August 2016, available online: http://www.tandfonline.com/10.1080/17435390.2016.1218080.
Keywords: Corona, lung, nanoparticles, surfactant, surface chemistry
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health > Neonatology
URI: https://discovery.ucl.ac.uk/id/eprint/1517302
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