O'Callaghan, AR;
Morgan, L;
Daniels, JT;
Lewis, MP;
(2016)
Human-derived feeder fibroblasts for the culture of epithelial cells for clinical use.
Regenerative Medicine
, 11
(6)
pp. 529-543.
10.2217/rme-2016-0039.
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Abstract
AIM: To investigate human oral mucosal fibroblasts (HOMF) and human limbal fibroblasts (HLF) as alternatives to murine 3T3 feeder fibroblasts currently used to support epithelial cell expansion for the treatment of limbal epithelial stem cell deficiency. METHODS: HLF and HOMF were compared with 3T3s for their ability to support the culture of human limbal epithelial cells and human oral mucosal epithelial cells. RESULTS: HOMF, but not HLF, were equivalent to 3T3s in terms of the number of epithelial population doublings achieved. Human limbal epithelial cells co-cultured with HOMF or 3T3s had similar expression of corneal and putative stem cell markers. CONCLUSION: HOMF are a suitable and safer feeder fibroblast alternative to 3T3s for the production of epithelial cells for clinical use.
| Type: | Article |
|---|---|
| Title: | Human-derived feeder fibroblasts for the culture of epithelial cells for clinical use |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.2217/rme-2016-0039 |
| Publisher version: | http://doi.org/10.2217/rme-2016-0039 |
| Language: | English |
| Additional information: | © 2016 Future Medicine Ltd. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Technology, Cell & Tissue Engineering, Engineering, Biomedical, Cell Biology, Engineering, cornea, limbal epithelial cells, limbal epithelial stem cell deficiency, limbal fibroblasts, oral mucosa, oral mucosal epithelial cells, oral mucosal fibroblasts, stem cell therapy, ORAL MUCOSAL EPITHELIUM, EX-VIVO EXPANSION, STEM-CELLS, HUMAN LIMBAL, OCULAR SURFACE, AMNIOTIC MEMBRANE, CORNEAL, SHEETS, NICHE, DIFFERENTIATION |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1508547 |
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