Lemech, CR;
Ensell, L;
Paterson, JC;
Eminowicz, G;
Lowe, H;
Arora, R;
Arkenau, H-T;
... Kristeleit, RS; + view all
(2016)
Enumeration and Molecular Characterisation of Circulating Tumour Cells in Endometrial Cancer.
Oncology
, 91
(1)
pp. 48-54.
10.1159/000445999.
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Abstract
BACKGROUND: This is a feasibility study to determine whether circulating tumour cells (CTCs) are detectable and suitable for molecular profiling in advanced endometrial cancer (aEC). METHOD: Between October 2012 and February 2014, 30 patients with aEC had baseline and up to 3 follow-up samples. CTCs and stathmin expression were evaluated using the CellSearch platform. Epithelial cell adhesion molecule (EpCAM) and stathmin immunohistochemistry were performed on FFPE tumour tissue. RESULTS: Eighteen from 30 (60%) patients had detectable CTCs during study [1 CTC (n = 7), 2 (n = 4), 3 (n = 1), 4 (n = 2), 7 (n = 1), 8 (n = 1), 22 (n = 1), 172 (n = 1) in 7.5 ml blood]. Ten from 18 patients had between 50 and 100% of detectable CTCs that were stathmin positive. More CTC-positive than CTC-negative patients had non-endometrioid versus endometrioid histology, tumour size ≥5 versus <5 cm, higher-stage disease and worse survival [hazard ratio 3.3, p > 0.05, 95% confidence interval 0.7-16.2]. Twenty-one tumour blocks were tested for EpCAM and stathmin immunohistochemistry (IHC). Stathmin tumour immunostaining scores (TIS) on IHC were higher in CTC-positive patients. CONCLUSION: CTC enumeration and molecular profiling with stathmin on the CellSearch platform is feasible in aEC. Stathmin TIS on IHC, a known prognostic marker in EC, was associated with CTC positivity.
| Type: | Article |
|---|---|
| Title: | Enumeration and Molecular Characterisation of Circulating Tumour Cells in Endometrial Cancer |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1159/000445999 |
| Publisher version: | http://dx.doi.org/10.1159/000445999 |
| Language: | English |
| Additional information: | Copyright © 2016 S. Karger AG, Basel. This is the peer-reviewed but unedited manuscript version of the following article: [Lemech, CR et al (2016) Enumeration and Molecular Characterisation of Circulating Tumour Cells in Endometrial Cancer. Oncology , 91 (1) pp. 48-54. 10.1159/000445999]. The final, published version is available at http://www.karger.com/?doi=10.1159/000445999 |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Oncology, Circulating tumour cells, Endometrial cancer, Stathmin, CellSearch, Epithelial cell adhesion molecule, Resistant Prostate-cancer, Breast-cancer, Poor-prognosis, Stathmin Expression, Adhesion Molecule, Colorectal-cancer, Peripheral-blood, Ovarian-cancer, Carcinoma, Survival |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > CRUK Cancer Trials Centre UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health > Womens Cancer |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1499891 |
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