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Enumeration and Molecular Characterisation of Circulating Tumour Cells in Endometrial Cancer

Lemech, CR; Ensell, L; Paterson, JC; Eminowicz, G; Lowe, H; Arora, R; Arkenau, H-T; ... Kristeleit, RS; + view all (2016) Enumeration and Molecular Characterisation of Circulating Tumour Cells in Endometrial Cancer. Oncology , 91 (1) pp. 48-54. 10.1159/000445999. Green open access

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Abstract

BACKGROUND: This is a feasibility study to determine whether circulating tumour cells (CTCs) are detectable and suitable for molecular profiling in advanced endometrial cancer (aEC). METHOD: Between October 2012 and February 2014, 30 patients with aEC had baseline and up to 3 follow-up samples. CTCs and stathmin expression were evaluated using the CellSearch platform. Epithelial cell adhesion molecule (EpCAM) and stathmin immunohistochemistry were performed on FFPE tumour tissue. RESULTS: Eighteen from 30 (60%) patients had detectable CTCs during study [1 CTC (n = 7), 2 (n = 4), 3 (n = 1), 4 (n = 2), 7 (n = 1), 8 (n = 1), 22 (n = 1), 172 (n = 1) in 7.5 ml blood]. Ten from 18 patients had between 50 and 100% of detectable CTCs that were stathmin positive. More CTC-positive than CTC-negative patients had non-endometrioid versus endometrioid histology, tumour size ≥5 versus <5 cm, higher-stage disease and worse survival [hazard ratio 3.3, p > 0.05, 95% confidence interval 0.7-16.2]. Twenty-one tumour blocks were tested for EpCAM and stathmin immunohistochemistry (IHC). Stathmin tumour immunostaining scores (TIS) on IHC were higher in CTC-positive patients. CONCLUSION: CTC enumeration and molecular profiling with stathmin on the CellSearch platform is feasible in aEC. Stathmin TIS on IHC, a known prognostic marker in EC, was associated with CTC positivity.

Type: Article
Title: Enumeration and Molecular Characterisation of Circulating Tumour Cells in Endometrial Cancer
Open access status: An open access version is available from UCL Discovery
DOI: 10.1159/000445999
Publisher version: http://dx.doi.org/10.1159/000445999
Language: English
Additional information: Copyright © 2016 S. Karger AG, Basel. This is the peer-reviewed but unedited manuscript version of the following article: [Lemech, CR et al (2016) Enumeration and Molecular Characterisation of Circulating Tumour Cells in Endometrial Cancer. Oncology , 91 (1) pp. 48-54. 10.1159/000445999]. The final, published version is available at http://www.karger.com/?doi=10.1159/000445999
Keywords: Science & Technology, Life Sciences & Biomedicine, Oncology, Circulating tumour cells, Endometrial cancer, Stathmin, CellSearch, Epithelial cell adhesion molecule, Resistant Prostate-cancer, Breast-cancer, Poor-prognosis, Stathmin Expression, Adhesion Molecule, Colorectal-cancer, Peripheral-blood, Ovarian-cancer, Carcinoma, Survival
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > CRUK Cancer Trials Centre
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst for Women's Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > Inst for Women's Health > Women's Cancer
URI: https://discovery.ucl.ac.uk/id/eprint/1499891
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